Leptin u zhenshchin, bol'nykh sakharnym diabetom2 tipa, ne poluchayushchikh lekarstvennuyusakharosnizhayushchuyu terapiyu
https://doi.org/10.14341/2071-8713-5277
Abstract
The aim of this study was to investigate the effect of acute hyperinsulinemia to fasting leptin level (fL) secretion and leptin
secretion during intravenouse glucose tolerance test (IVGTT). Methods. 32 T2DM women without antidiabetic drugs were studied.
Median age of participants was 57,5[50,0;62,5] years, median body mass index (BMI) was 32,7[29,1;37,1]. Glucose intravenouse
bolus solution (40%) was dosing (0,75 g/kg BM). Insulin level was investigated in fasting state (FI), 2 min, 70 min and 120 min after
glucose loading. Leptin was investigated in fasting (FL) and 120 min after glucose loading. Results. FL level was 21,1[13,6;39,0]
ng/ml. Relationships between FL and HOMA-R, аnd between FL and FI were enough strong (r=0,5, р<0,05). FL in the patient
with moderate diabetes control was twice as much compared to FL in patients with poor diabetes control: (28,0[16,8;47,5] v.s.
12,6[9,2;14,3] ng/ml, (р<0,05)). We found a significant decrease in leptin level at 120 min IVGTT (11% from FL, (р<0,05)). The
most reduction of leptin level was in moderate diabetes control group, which had the overall area under insulin curve. We discovered
a correlation between percentage of leptin reduction and insulin increase on 70 minute of IVGTT (r=0,5, р<0,05). Inverse correlation
was found between percent of leptin reduction in IVGTT and high density lipoprotein level (r=-0,4, р<0,02). Conclusion. FL in
moderate diabetes control group was two times higher compared to poor diabetes control group. Leptin level after glucose loading
was decreased compared with FL.
secretion during intravenouse glucose tolerance test (IVGTT). Methods. 32 T2DM women without antidiabetic drugs were studied.
Median age of participants was 57,5[50,0;62,5] years, median body mass index (BMI) was 32,7[29,1;37,1]. Glucose intravenouse
bolus solution (40%) was dosing (0,75 g/kg BM). Insulin level was investigated in fasting state (FI), 2 min, 70 min and 120 min after
glucose loading. Leptin was investigated in fasting (FL) and 120 min after glucose loading. Results. FL level was 21,1[13,6;39,0]
ng/ml. Relationships between FL and HOMA-R, аnd between FL and FI were enough strong (r=0,5, р<0,05). FL in the patient
with moderate diabetes control was twice as much compared to FL in patients with poor diabetes control: (28,0[16,8;47,5] v.s.
12,6[9,2;14,3] ng/ml, (р<0,05)). We found a significant decrease in leptin level at 120 min IVGTT (11% from FL, (р<0,05)). The
most reduction of leptin level was in moderate diabetes control group, which had the overall area under insulin curve. We discovered
a correlation between percentage of leptin reduction and insulin increase on 70 minute of IVGTT (r=0,5, р<0,05). Inverse correlation
was found between percent of leptin reduction in IVGTT and high density lipoprotein level (r=-0,4, р<0,02). Conclusion. FL in
moderate diabetes control group was two times higher compared to poor diabetes control group. Leptin level after glucose loading
was decreased compared with FL.
References
1. Дедов И.И., Мельниченко Г.А. Ожирение. - М.: МИА, 2004, С. 53-62.
2. Древаль А.В. Двумерный параметр кинетики глюкозы в диагностике сахарного диабета // Лабораторное дело, 1988; С. 4: 47-54.
3. Bates S.H., Gardiner J.V., Jones R.B., Bloom S.R., Bailey C.J. Acute Stimulation of Glucose Uptake by Leptin in L6 Muscle Cells // Horm. Metab. Res., 2002; 34: Р. 111-5.
4. Bergen H., Funabashi T., Kleopoulos S.P., Zhong Y.G., Bauman W.A., Mobbs C.V. Obese gene expression: reduction by fasting and stimulation by insulin and glucose in lean mice, and persistent elevation in acquired (diet-induced) and genetic (yellow agouti) obesity // Proc. Natl. Acad. Sci. USA, 1996; 93: Р. 3434-8.
5. Buyukbese M.A., Cetinkaya A., Kocabas R., Guven A., Tarakcioglu M. Leptin levels in obese women with and without type 2 diabetes mellitus // Cell Signal, 2002; 14(8): Р. 655-63.
6. Casabiell X., Pineiro V., Peino R., et al. Gender differences in both spontaneous and stimulated leptin secretion by human omental adipose tissue in vitro: dexamethasone and estradiol stimulate leptin release in women, but not in men // J. Clin. Endocrinol. Metab., 1998; 83: Р. 2149-55.
7. Clement K., Lahlou N., Ruiz J. et al. Association of poorly controlled diabetes with low serum leptin in morbid obesity // Int. J. Obes. Relat. Metab. Disord., 1997; 21: Р. 556-61.
8. Considine R.V., Sinha M.K., Heiman M.L., Kriauciunas A., Stephens T.W., Nyce M.R., Ohannesian J.P., Marco C.C., McKee L.J., Bauer T.L. Serum immunoreactive- leptin concentrations in normal-weight and obese humans // N. Engl. J. Med., 1996; 334: Р. 292-5.
9. Flanagan D.E., Vaile J.C. Gender differences in the relationship between leptin, insulin resistance and the autonomic nervous system // Regulatory Peptides, 2007, 140: Р. 37-42.
10. Fried S.K., Ricci M.R., Russell C.D., et al. Regulation of leptin production in humans // J. Nutr., 2000: 130: 3127S-31S.
11. Fruehwald-Schultes B., Oltmanns K.M., Kern W., Born J., Fehm H.L., Peters A. The effect of experimentally induced insulin resistance on the leptin response to hyperinsulinaemia // Int. J. Obes. Relat. Metab. Disord., 2002; 26: Р. 510- 6.
12. Iritani N., Sugimoto T., Fukuda H. Gene expressions of leptin, insulin receptors and lipogenic enzymes are coordinately regulated by insulin and dietary fat in rats // J. Nutr., 130: Р. 1183-88, 2000.
13. Maffei M., Halaas J., Ravussin E., Pratley R.E., Lee G.H., Zhang Y., Fei H., Kim S., Lallone R., Ranganathan S. Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects // Nat. Med., 1995; 1: Р. 1155-61.
14. Meier U., Gressner A.M. Endocrine Regulation of Energy Metabolism: Review of Pathobiochemical and Clinical Chemical Aspects of Leptin, Ghrelin, Adiponectin, and Resistin // Clinical Chemistry, 2004; 50: Р. 1511-5. OM#1-2010_08_Blok 4.6.10 10:10 AM Page 44
15. Ostlund R.J., Yang J.W., Klein S., Gingerich R. Relation between plasma leptin concentration and body fat, gender, diet, age, and metabolic covariates // Journal of Clinical Endocrinology and Metabolism, 1996; 81: Р. 3909-13.
16. Sandhofer A., Laimer M., Ebenbichler C.F., Kaser S., Paulweber B., Patsch J.R. Soluble leptin receptor and soluble receptor-bound fraction of leptin in the metabolic syndrome // Obes. Res., 2003; 11: Р. 760-68.
17. Schwartz M.W., Woods S.C., Porte D., Seeley R.J., Baskin D.G. Central nervous system control of food intake // Nature, 2000; 404: Р. 661-71.
18. Sivitz W.I., Walsh S.A., Morgen D.A., Thomas M.J., Haynes W.G. Effects of leptin on insulin sensitivity in normal rats // Endocrinology, 1997; 138: Р. 3395-401.
19. Unger R. Lipotoxic Diseases // Annu. Rev. Med., 2002; 53: Р. 319-36.
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, , Leptin u zhenshchin, bol'nykh sakharnym diabetom2 tipa, ne poluchayushchikh lekarstvennuyusakharosnizhayushchuyu terapiyu. Obesity and metabolism. 2010;7(1):41-45. (In Russ.) https://doi.org/10.14341/2071-8713-5277
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