ometendoОжирение и метаболизмObesity and metabolism2071-87132306-5524Endocrinology Research Centre10.14341/2071-8713-5083ometendo-5083Research ArticleСтатьиArticlesУровень висфатина сыворотки крови и экспрессиягена висфатина (PBEF1) в подкожнойи висцеральной жировой ткани у детейUroven' visfatina syvorotki krovi i ekspressiyagena visfatina (PBEF1) v podkozhnoyi vistseral'noy zhirovoy tkani u deteyKosyginaA V-SosunovV V-PeterkovaV A-DedovI I-20101512201074№4 (2010)2023Copyright © Kosygina A.V., Sosunov V.V., Peterkova V.A., Dedov I.I., 20102010Kosygina A.V., Sosunov V.V., Peterkova V.A., Dedov I.I.Kosygina A.V., Sosunov V.V., Peterkova V.A., Dedov I.I.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.omet-endojournals.ru/jour/article/view/5083

Целью проведенной работы являлось исследование особенностей экспрессии гена висфатина - PBEF1 - в подкожной и висцеральной жировой ткани и содержания висфатина в сыворотке крови у детей, а также выявление взаимосвязи между изучаемыми факторами и основными антропометрическими показателями. Обследованы 52 пациента (27 мальчиков (52%), 25 девочек (48%) в возрасте от 2,5 до 18 лет (13,8 [7,4-15,1] лет)), подвергшихся плановым оперативным вмешательствам. Проводилось определение экспрессии гена PBEF1 в парных образцах жировой ткани методом ПЦР в «реальном времени», определение уровня висфатина сыворотки крови. По данным нашего исследования, сывороточная концентрация висфатина в группе детей с нормальной массой тела составила 9,2 [7,4-11,6] нг/мл, а в группе с избыточной массой тела - 9,5 [7,5-11,1] нг/мл (р=0,5). Не выявлено статистически значимых различий содержания висфатина в зависимости от пола обследованных детей. Не было обнаружено корреляционных взаимосвязей между сывороточным содержанием висфатина, возрастом и основными антропометрическими показателями. Выявлено преобладание экспрессии гена PBEF1 в висцеральной жировой ткани (р=0,002) в группе всех обследованных детей и группе детей с нормальной массой тела, в то время как у детей с избыточной массой тела ген висфатина экспрессировался без значимых депо-специфических отличий. Проведенное исследование не выявило зависимости экспрессии гена от возраста и пола обследованных детей. Экспрессия гена PBEF1 в жировой ткани снижается по мере полового созревания (PBEF1ВЖТγ=-0,24, р=0,02; PBEF1ПЖТγ=-0,25, р=0,02). Содержание висфатина в сыворотке крови не взаимосвязано с уровнем экспрессии гена PBEF1 в жировой ткани (R=-0,06, р=0,6).

The aim of this study was to investigate the characteristics of visfatin - PBEF1 - gene expression in subcutaneous and visceral adipose tissue and serum levels of visfatin in children with relation to age and anthropometric parameters. The study included 52 patients (27 boys (52%), 25 girls (48%) aged from 2,5 to 18 years (13,8 [7,4-15,1] years)), who underwent an elective surgical intervention. PBEF1 mRNA level was measured by real-time PCR and serum level of visfatin was quantified by immunoenzyme assay. According to our study visfatin serum concentration in children with normal body weight was 9,2 [7,4-11,6] ng/ml, whereas in the overweight group - 9,5 [7,5-11,1] ng/ml (p=0,5). No statistically significant gender difference in serum visfatin levels was observed. No correlation between visfatin levels and age, pubertal stages and anthropometric indices in children was found. Statistically significant differences in the level of gene expression between subcutaneous and visceral adipose tissue were found (р=0,002) in the total group of children surveyed and children with normal weight, while there were no depot-specific differences in overweight children. The study did not reveal any dependence of PBEF1 expression on age and sex of children. Expression of PBEF1 in adipose tissue decreases with puberty (PBEF1VATγ=-0,24, р=0,02; PBEF1SATγ=-0,25, р=0,02). PBEF1 expression in adipose tissue was not correlated with the serum visfatin (R=-0,06, р=0,6).

висфатинжировая тканьэкспрессия геновдетиподросткиvisfatinadipose tissuegene expressionchildrenadolescentsReferencesBerndt J., Kloting N., Kralisch S. et al. Plasma visfatin concentrations and fat depot-specific mRNA expression in humans // Diabetes 2005; 54: 2911- 2916.Berndt J., Kloting N., Kralisch S. et al. Plasma visfatin concentrations and fat depot-specific mRNA expression in humans // Diabetes 2005; 54: 2911- 2916.Davutoglua M., Ozkayab M. et al. Plasma visfatin concentrations in childhood obesity: relationships to insulin resistance and anthropometric indi - ces // Swiss. Med. Wkly 2009; 139 (1-2): 22-27.Davutoglua M., Ozkayab M. et al. Plasma visfatin concentrations in childhood obesity: relationships to insulin resistance and anthropometric indi - ces // Swiss. Med. Wkly 2009; 139 (1-2): 22-27.Dedoussis G.V., Kapiri A., Samara A. et al. Visfatin: the link between inflammation and childhood obesity. // Diabetes Care 2009; 32(6): 71.Dedoussis G.V., Kapiri A., Samara A. et al. Visfatin: the link between inflammation and childhood obesity. // Diabetes Care 2009; 32(6): 71.Fukuhara A., Matsuda M., Nishizawa M. et al. Visfatin: a protein secreted by visceral fat that mimics the effects of insulin // Science 2005; 307: 426-30.Fukuhara A., Matsuda M., Nishizawa M. et al. Visfatin: a protein secreted by visceral fat that mimics the effects of insulin // Science 2005; 307: 426-30.Kim M.K., Lee J.H., Kim H. et al. Crystal structure of visfatin/pre-B cell colonyenhancing factor 1/nicotinamide phosphoribosyltransferase, free and in complex with the anti-cancer agent FK-866 // J. Mol. Biol. 2006; 362: 66-77.Kim M.K., Lee J.H., Kim H. et al. Crystal structure of visfatin/pre-B cell colonyenhancing factor 1/nicotinamide phosphoribosyltransferase, free and in complex with the anti-cancer agent FK-866 // J. Mol. Biol. 2006; 362: 66-77.Kovacikova M., Vitkova M., Klimcakova E. et al. Visfatin expression in subcutaneous adipose tissue of pre-menopausal women: relation to hormones and weight reduction // Eur. J. Clin. Invest 2008; 38: 516-522.Kovacikova M., Vitkova M., Klimcakova E. et al. Visfatin expression in subcutaneous adipose tissue of pre-menopausal women: relation to hormones and weight reduction // Eur. J. Clin. Invest 2008; 38: 516-522.Martin P.R., Shea R.J., Mulks M.H. Identification of a plasmid-encoded gene from Haemo - philus ducreyi which confers NAD independence // J. Bacteriol. 2001; 183: 1168-74.Martin P.R., Shea R.J., Mulks M.H. Identification of a plasmid-encoded gene from Haemo - philus ducreyi which confers NAD independence // J. Bacteriol. 2001; 183: 1168-74.Rongvaux A., Shea R.J., Mulks M.H., Gigot D., Urbain J., Leo O., Andris F. Pre-Bcell colony-enhancing factor, whose expression is up-regulated in activated lymphocytes, is a nicotinamide phosphoribosyltransferase, a cytosolic enzyme involved in NAD biosynthesis // Eur. J. Immunol. 2002; 32: 3225-34.Rongvaux A., Shea R.J., Mulks M.H., Gigot D., Urbain J., Leo O., Andris F. Pre-Bcell colony-enhancing factor, whose expression is up-regulated in activated lymphocytes, is a nicotinamide phosphoribosyltransferase, a cytosolic enzyme involved in NAD biosynthesis // Eur. J. Immunol. 2002; 32: 3225-34.Samal B. et al. Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor // Mol. Cell. Biol. 1994; 14(2): 1431-7.Samal B. et al. Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor // Mol. Cell. Biol. 1994; 14(2): 1431-7.Sommer G. et al. Visfatin/PBEF/Nampt: structure, regulation and potential function of a novel adipokine // Clinical Science 2008; 115: 13-23.Sommer G. et al. Visfatin/PBEF/Nampt: structure, regulation and potential function of a novel adipokine // Clinical Science 2008; 115: 13-23.Varma V., Yao-Borengasser A., Rasouli N. et al. Human visfatin expression: relationship to insulin sensitivity, intramyocellular lipids, and inflammation // J. Clin. Endocrinol. Metab. 2007; 92: 666-672.Varma V., Yao-Borengasser A., Rasouli N. et al. Human visfatin expression: relationship to insulin sensitivity, intramyocellular lipids, and inflammation // J. Clin. Endocrinol. Metab. 2007; 92: 666-672.Wang T., Zhang X., Bheda P., Revollo J.R., Imai S., Wolberger C. Structure of Nampt/PBEF/visfatin, a mammalian NAD+ biosynthetic enzyme // Nat. Struct. Mol. Biol. 2006; 13: 661-662.Wang T., Zhang X., Bheda P., Revollo J.R., Imai S., Wolberger C. Structure of Nampt/PBEF/visfatin, a mammalian NAD+ biosynthetic enzyme // Nat. Struct. Mol. Biol. 2006; 13: 661-662.

The authors declare that there are no conflicts of interest present.