ometendoОжирение и метаболизмObesity and metabolism2071-87132306-5524Endocrinology Research Centre10.14341/omet12938ometendo-12938Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL STUDIESАссоциации индекса массы тела с уровнем свободных жирных кислот у мужчинAssociations of body mass index with the level of free fatty acids in menhttps://orcid.org/0000-0002-0436-2549ШрамкоВ. С.ShramkoV. S.

Шрамко Виктория Сергеевна - к.м.н.

630089, Новосибирск, ул. Бориса Богаткова, д. 175/1

ResearcherID ABG-9543-2020; Scopus Author ID 57194556107

Viktoriya S. Shramko - MD, PhD.

175/1 Bogatkov street, 630089 Novosibirsk

ResearcherID ABG-9543-2020; Scopus Author ID 57194556107

Nosova@211.ruhttps://orcid.org/0000-0003-2268-4186КаштановаЕ. В.KashtanovaE. V.

Каштанова Елена Владимировна - д.б.н.

Новосибирск

Researcher ID J-4675-2016; Scopus Author ID 8645249000

Elena V. Kashtanova - MD, PhD in biology.

Novosibirsk

Researcher ID J-4675-2016; Scopus Author ID 8645249000

elekastanova@yandex.ruhttps://orcid.org/0000-0001-9270-9188ЩербаковаЛ. В.ShcherbakovaL. V.

Щербакова Лилия Валерьевна.

Новосибирск

Researcher ID ABA-9069-2021; Scopus Author ID 15030341800

Liliya V. Shcherbakova.

Novosibirsk

Researcher ID ABA-9069-2021; Scopus Author ID 15030341800

9584792@mail.ruhttps://orcid.org/0000-0002-3538-0280ПолонскаяЯ. В.PolonskayaYa. V.

Полонская Яна Владимировна - д.б.н.

Новосибирск

Researcher ID H-4397-2016; Scopus Author ID 57216801775

Yana V. Polonskaya - MD, PhD in biology.

Novosibirsk

Researcher ID H-4397-2016; Scopus Author ID 57216801775

yana-polonskaya@yandex.ruhttps://orcid.org/0000-0003-0484-6540СтахнёваЕ. М.StakhnevaE. M.

Стахнёва Екатерина Михайловна - к.б.н.

Новосибирск

Researcher ID R-1589-2016; Scopus Author ID 24774022600

Ekaterina M. Stakhneva - PhD in biology.

Novosibirsk

Researcher ID R-1589-2016; Scopus Author ID 24774022600

stahneva@yandex.ruhttps://orcid.org/0000-0002-4936-8362РагиноЮ. И.RaginoYu. I.

Рагино Юлия Игоревна - д.м.н., профессор.

Новосибирск

ResearcherID E-9498-2015; Scopus Author ID 6602418274

Yulia I. Ragino - MD, PhD, Professor.

Novosibirsk

ResearcherID E-9498-2015; Scopus Author ID 6602418274

ragino@mail.ruНаучно-исследовательский институт терапии и профилактической медициныРоссияResearch Institute of Internal and Preventive MedicineRussian Federation202417062024213252262Copyright © Шрамко В.С., Каштанова Е.В., Щербакова Л.В., Полонская Я.В., Стахнёва Е.М., Рагино Ю.И., 20242024Шрамко В.С., Каштанова Е.В., Щербакова Л.В., Полонская Я.В., Стахнёва Е.М., Рагино Ю.И.Shramko V.S., Kashtanova E.V., Shcherbakova L.V., Polonskaya Y.V., Stakhneva E.M., Ragino Y.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.omet-endojournals.ru/jour/article/view/12938Обоснование

Обоснование. У людей с избыточным количеством жировой ткани наблюдаются повышенные уровни свободных жирных кислот (СЖК) в крови, что в итоге приводит к нарушениям метаболизма липидов и резистентности к инсулину, которые являются основными факторами в развитии сахарного диабета.

Цель

Цель. Изучить содержание СЖК в плазме крови, а также их ассоциации с массой тела у мужчин.

Материалы и методы

Материалы и методы. Проведено одноцентровое обсервационное одномоментное исследование. Выборка формировалась случайным репрезентативным методом, сопоставимая по полу и возрасту. В плазме крови определяли уровни СЖК методом высокоэффективной жидкостной хроматографии с масс-спектрометрией.

Результаты

Результаты. В исследование включены 250 мужчин. Отобранные участники были разделены на группы согласно их индексу массы тела (ИМТ): группа 1 — 62 человека с ИМТ≤24,9 кг/м2, группа 2 — 101 человек с ИМТ 25,0–29,9 кг/м2, группа 3 — 87 человек с ИМТ≥30,0 кг/м2. Мужчин с ожирением разделили на группу 4 — 62 человека с ИМТ 30,0–34,9 кг/м2, группу 5 — 19 человек с ИМТ 35,0–39,9 кг/м2, группу 6 — 6 человек с ИМТ≥40,0 кг/м2.

Содержание докозатетраеновой кислоты было выше в группах 2 (р=0,002) и 5 (р=0,003) при сравнении с группой 1. Содержание гамма-линоленовой кислоты было выше в группе 3, чем в группе 1 (р=0,041). Уровни олеиновой, линолевой, дигомо-гамма-линоленовой, мидовой, арахидоновой, эйкозапентаеновой кислот были выше в группе 5, чем в группе 1 (р=0,007, р=0,023, р=0,004, р=0,019, р=0,006, р=0,001 соответственно), а также чем в группе 2 (р=0,006, р=0,017, р=0,007, р=0,007, р=0,008, р=0,001 соответственно). Содержание нервоновой кислоты выше в группах 1 (р=0,029) и 2 (р=0,012), чем в группе 4. Ожирение ассоциировано с повышением уровня гамма-линоленовой (1,030, 1,006–1,056, р=0,015) и эйкозапентаеновой кислотами (1,061, 1,000–1,125, р=0,045) и снижением уровня нервоновой кислоты (0,953, 0,913–0,994, р=0,027).

Заключение

Заключение. Уровни СЖК плазмы крови значительно отличаются у мужчин с нормальной массой тела и наличием ожирения. Содержание олеиновой, гамма-линоленовой, мидовой, дигомо-гамма-линоленовой, арахидоновой, докозатетраеновой и эйкозапентаеновой ЖК были значительно выше у мужчин с ожирением 2 степени. Увеличение уровня гамма-линоленовой и эйкозапентаеновой кислот и снижение уровня нервоновой кислоты ассоциированы с ожирением независимо от возраста.

BACKGROUND

BACKGROUND: The people with an excessive amount of adipose tissue have elevated levels of free fatty acids (FFA) in the blood, which ultimately leads to disorders of lipid metabolism and insulin resistance, which are the main factors in the development of diabetes mellitus.

AIM

AIM: To study the content of FFA in blood plasma, as well as their association with body weight in men.

MATERIALS AND METHODS

MATERIALS AND METHODS: A single-center observational one-stage study was conducted. The sample was formed by a random representative method, comparable by sex and age. The levels of FFA were determined in the blood plasma by high-performance liquid chromatography with mass spectrometry.

RESULTS

RESULTS: The study included 250 men. The selected participants were divided into groups according to their body mass index (BMI): Group 1 — 62 people with BMI≤24.9 kg/m2, Group 2 — 101 people with BMI 25.0–29.9 kg/m2, Group 3 — 87 people with BMI≥30.0 kg/m2. Obese men were divided into: Group 4 — 62 people with BMI 30.0–34.9 kg/m2, Group 5 — 19 people with BMI 35.0–39.9 kg/m2, Group 6 — 6 people with BMI≥40.0 kg/m2.

The content of docosatetraenoic acid was higher in groups 2 (p=0.002) and 5 (p=0.003), when compared with group 1. The content of gamma-linolenic acid was higher in group 3 than in group 1 (p=0.041). Concentration of oleic; linoleic; dihomo-gamma-linolenic; midic; arachidonic; eicosapentaenoic acids were higher in group 5 than in group 1 (p=0.007, p=0.023, p=0.004, p=0.019, p=0.006, p=0.001, respectively), and also than in group 2 (p=0.006, p=0.017, p=0.007, p=0.007, p=0.008, p=0.001, respectively). The content of nervonic acid is higher in groups 1 (p=0.029) and 2 (p=0.012) than in group 4. Obesity is associated with increased levels of gamma-linolenic (1.030, 1.006–1.056, p=0.015) and eicosapentaenoic acids (1.061, 1.000–1.125, p=0.045), and a decrease in the level of nervonic acid (0.953, 0.913–0.994, p=0.027).

CONCLUSION

CONCLUSION: The FFA levels of blood plasma is significantly different in men with normal body weight and the presence of obesity. The content of oleic, gamma-linolenic, midic, digomo-gamma-linolenic, arachidonic, docosatetraenoic and ­eicosapentaenoic acids was significantly higher in men with grade 2 obesity. An increase in the level of gamma-linolenic and eicosapentaenoic acids, and a decrease in the level of nervonic acid are associated with obesity, regardless of the age.

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The authors declare that there are no conflicts of interest present.