ometendoОжирение и метаболизмObesity and metabolism2071-87132306-5524Endocrinology Research Centre10.14341/omet10189ometendo-10189Research ArticleНаучные исследованияOriginal studiesАссоциация полиморфизма rs2167270 гена лептина (LEP) с интенсивностью боли у больных остеоартритом коленного суставаAssociation of the rs2167270 polymorphism of the leptin gene (LEP) with the intensity of pain in patients with osteoarthritis of the kneehttps://orcid.org/0000-0002-9922-5124КрыловМ. Ю.KrylovM. Yu.

Крылов Михаил Юрьевич - кандидат медицинских наук; Researcher ID: N-6239-2019; Scopus Author ID: 920397006951088; eLibrary SPIN: 2696-7661.

115522, Москва, Каширское шоссе, д. 34А

Mikhail Yu. Krylov, MD, PhD; Researcher ID: N-6239-2019; Scopus Author ID: 920397006951088; eLibrary SPIN: 2696-7661.

Kashirskoe shosse 34A, 115522 Moscow

mekry@yandex.ruhttps://orcid.org/0000-0002-8931-4991АлексееваЛ. И.AlekseevaL. I.

Алексеева Людмила Ивановна - доктор медицинских наук, профессор; eLibrary SPIN: 4714-8572

Москва

Ludmila I. Alekseeva, MD, PhD, Professor; eLibrary SPIN: 4714-8572.

Moscow

dr.alekseeva@mail.comhttps://orcid.org/0000-0003-2048-5183ШараповаЕ. П.ScharapovaE. P.

Шарапова Евгения Павловна, кандидат медицинских наук; eLibrary SPIN: 9994-0234.

Москва

Eugenia P. Scharapova MD, PhD, researcher; eLibrary SPIN: 9994-0234.

Moscow

2116i@mail.ruНаучно-исследовательский институт ревматологии имени В.А. НасоновойРоссияV.A. Nasonova Research Institute of RheumatologyRussian Federation202124072021182210217Copyright © Крылов М.Ю., Алексеева Л.И., Шарапова Е.П., 20212021Крылов М.Ю., Алексеева Л.И., Шарапова Е.П.Krylov M.Y., Alekseeva L.I., Scharapova E.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.omet-endojournals.ru/jour/article/view/10189Обоснование

Обоснование. Остеоартрит (ОА) — значимая социальная проблема, так как является самым частым заболеванием суставов. ОА является многофакторным заболеванием, при котором большое внимание уделяется роли наследственных факторов. В последнее время ряд исследований продемонстрировал вклад ряда генов в субъективную оценку боли при ОА, которая является главным симптомом этого заболевания. Была показана связь генов P2X7, TRPV1 и TACR1 и некоторых других с чувствительностью к боли. Одним из факторов риска возникновения боли, среди многих других, является повышенный вес. Абдоминальная жировая ткань является источником высвобождения провоспалительных адипокинов, вызывающих системное воспаление, связанное с повреждением многих тканей, включая субхондральную кость, синовиальную оболочку. Лептин является эндогенным гормоном из семейства адипокинов, кодируемым геном ожирения лептином (LEP), который синтезируется главным образом в адипоцитах. Роль гена LEP в субъективной оценке боли при ОА в отечественных исследованиях не изучалась.

Цель

Цель. Изучить возможную связь полиморфизма rs2167270 (A19G) гена LEP с интенсивностью боли у больных ОА коленного сустава.

Материалы и методы

Материалы и методы. Исследование проведено среди женщин с диагнозом ОА. Используя шкалу ВАШ (Визуальная аналоговая шкала) для оценки субъективной боли самим пациентом, были изучены пациентки со слабой болью в коленном ­суставе — группа 1 (индекс ВАШ ≤40 мм) и пациентки с умеренной или выраженной болью — группа 2 (индекс ВАШ >40 мм). Генетические варианты A19G полиморфизма гена лептина были изучены с помощью полимеразной цепной реакции с последующим анализом длин рестриктных фрагментов (ПЦР-ПДРФ метод).

Результаты

Результаты. В группе пациентов с умеренной или выраженной интенсивностью боли (группа 2, n=61) по сравнению с группой 1 (n=36) показана статистически значимая связь с высоким индексом массы тела (р=0,006). В этой группе пациентов выявлена повышенная частота носителей 19GG генотипа (р=0,051) по сравнению с группой 1. Носители 19GG генотипа статистически значимо имели более высокий показатель ВАШ в коленном суставе и ранний возраст дебюта ОА по сравнению с носителями генотипа 19AA (р=0,035 и р=0,015 соответственно).

Заключение

Заключение. Полученные данные открывают новые возможности прогнозирования болевого симптома у больных ОА коленного сустава путем генетического тестирования A19G полиморфных вариантов гена LEP.

Background

Background: Osteoarthritis (OA) is a significant social problem as it is the most common disease of the joints. OA is a multifactorial disease in which great attention is paid to hereditary factors. Recently, a number of studies have demonstrated the contribution of a number of genes to the subjective assessment of pain in OA, which is the main symptom of this disease. The association of P2X7, TRPV1 and TACR1 genes and some others with pain sensitivity has been shown. One of the risk factors of pain among many others, is the increased weight. Abdominal adipose tissue is a source of release of pro-inflammatory adipokines that cause systemic inflammation associated with damage to many tissues, including subchondral bone, synovial membrane. Leptin is an endogenous hormone from the adipokine family encoded by the obesity gene leptin (LEP) and which is synthesized primarily in adipocytes.

Aims

Aims: To investigate the possible association of rs2167270 (A19G) polymorphism of the LEP gene with pain intensity in ­patients with knee OA.

Materials and methods

Materials and methods: The study was conducted among women diagnosed with OA. Using the VAS scale (Visual analog scale), patients with mild knee pain — group 1 (VAS ≤ 40 mm) and patients with moderate or severe pain — group 2 (VAS>40 mm) were selected for pain assessment. Genetic variants of A19G leptin gene polymorphism were studied by polymerase chain reaction followed by restriction fragment length analysis (PCR-RFLP) method.

Results

Results: In the group of patients with moderate or severe pain intensity (group 2, n=61), a statistically significant association was shown with a higher body mass index (p=0.006) and an increased frequency of carriers of the 19GG genotype (p=0,051) compared to group 1 (n=36). Carriers of the 19GG genotype statistically significantly had a higher rate of knee pain and an early age of OA debut compared to carriers of the 19AA genotype (p=0,035 and p=0,015, respectively).

Conclusions

Conclusions: The findings open up new possibilities for predicting pain symptoms in patients with knee OA by genetic testing of A19G polymorphic variants of the leptin gene.

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The authors declare that there are no conflicts of interest present.